Format

Send to

Choose Destination
Biophys J. 2004 Apr;86(4):2008-16.

Metastable network model of protein transport through nuclear pores.

Author information

1
Department of Physics, Bar Ilan University, Ramat-Gan 52900, Israel.

Abstract

To reconcile the observed selectivity and the high rate of translocation of cargo-importin complexes through nuclear pores, we propose that the core of the nuclear pore complex is blocked by a metastable network of phenylalanine and glycine nucleoporins. Although the network arrests the unfacilitated passage of objects larger than its mesh size, cargo-importin complexes act as catalysts that reduce the free energy barrier between the cross-linked and the dissociated states of the Nups, and open the network. Using Brownian dynamics simulations we calculate the distribution of passage times through the network for inert particles and cargo-importin complexes of different sizes and discuss the implications of our results for experiments on translocation of proteins through the nuclear pore complex.

PMID:
15041643
PMCID:
PMC1304054
DOI:
10.1016/S0006-3495(04)74262-9
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Elsevier Science Icon for PubMed Central
Loading ...
Support Center