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Opt Lett. 2004 Mar 15;29(6):584-6.

Time-resolved fluorescence anisotropy imaging applied to live cells.

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Departments of Physics, Biological Sciences and Chemistry, Imperial College London, London SW7 2BW, UK.


We have developed a wide-field time-resolved imaging system to image quantitatively both the fluorescence lifetime and the rotational correlation time of a fluorophore. Using a polarization-resolved imager, we simultaneously image orthogonal polarization components of the fluorescence emission onto a time-gated intensified CCD. We demonstrate imaging of solvent viscosity variations through the rotational correlation time of fluorescein in a multiwell plate and apply this technique to probe the microviscosity in live cells.

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