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J Am Chem Soc. 2004 Mar 24;126(11):3392-3.

Nitrilotriacetic acid-modified magnetic nanoparticles as a general agent to bind histidine-tagged proteins.

Author information

1
Department of Chemistry, Department of Physics, and Bioengineering Program, The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong, China. chbingxu@ust.hk

Abstract

Using Nalpha,Nalpha-bis(carboxymethyl)lysine to react with FePt magnetic nanoparticles, we synthesized the FePt-NTA conjugate, which immobilizes Ni2+ ions and selectively binds to histidine-tagged proteins at concentration as low as 0.5 pM. This simple system serves as a useful alternative to existing protocols for protein separation and also acts as a versatile agent for transporting and anchoring proteins.

PMID:
15025444
DOI:
10.1021/ja031776d
[Indexed for MEDLINE]

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