(A) Consensus motifs detected within 3′-UTR sequences of Puf3p, Puf4p, and Puf5p target mRNAs. Height of the letters specifies the probability of appearing at the position in the motif. Letters with less than 10% appearance were omitted. Fraction of genes bearing a motif in the 3′-UTR sequence is indicated to the right. Y-helicase proteins are nearly identical in sequence and were excluded from this analysis.
(B) Scheme of three-hybrid assay for monitoring RNA–protein interactions in vivo ().
(C) β-Galactosidase activity for three-hybrid assay. Proteins assayed are indicated on top, RNAs to the left. Abbreviations: pum, pum-HD; cons., consensus motif; UGU/AGA, UGU in consensus sequence mutated to AGA.
(D) Activation of HIS3 reporter gene and resistance to 3-aminotriazole (3-AT), a competitive inhibitor of the HIS3 gene product, in a three-hybrid assay ().