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Biochem Biophys Res Commun. 2004 Apr 2;316(2):588-93.

Actin filaments play an essential role for transport of nascent HIV-1 proteins in host cells.

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Division of Molecular Cell Biology, Institute of DNA Medicine, The Jikei University School of Medicine, Minato-ku, Tokyo 105-8461, Japan.


To investigate the role of actin filaments (F-actin) for human immunodeficiency virus type 1 (HIV-1) production in host cells, the effect of mycalolide B that is a novel actin-depolymerizing marine toxin was examined. Mycalolide B blocked the production of HIV-1 from primary infected T-lymphoblastoid and clonically infected monocytoid cells in a concentration-dependent manner. In the presence of 10 microM of mycalolide B, F-actins were disorganized and mostly disappeared in the host cells, and viral envelope- and capsid-proteins did not reach the plasma membrane, but were distributed in the cytoplasm forming aggregates. In electron micrographs, no HIV-1 virions were detected on the cell surface, but many lysosome-like vesicles containing electron dense granules were observed in the cytoplasm, implying that mycalolide B did not disturb the synthesis of viral proteins, but rather inhibited their transport processes of HIV-1 in the host cells.

[Indexed for MEDLINE]

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