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Methods Mol Biol. 2004;252:493-500.

Gene-array analysis of glioma cells after treatment with an anti-PKCalpha siRNA: a general protocol.

Author information

1
Department of Immunology, Molecular Medicine Group, The Norwegian Radium Hospital, Oslo.

Abstract

Post-transcriptional gene silencing is a powerful tool to reveal gene function. In this chapter, we have used gene-array technology to analyze changes in gene expression after specific targeting of the protein kinase Calpha isoform with small interfering RNAs (siRNAs). Expression profiles were investigated on a human gene array of 588 key genes involved in DNA synthesis, apoptosis, cell-cell communication, intracellular signal-transduction pathways, cell-surface molecules and transcription factors. The data indicate that siRNAs are an ideal tool for target validation, and show that RNA-dependent RNA polymerase is not required for RNAi in mammalian cells.

PMID:
15017074
DOI:
10.1385/1-59259-746-7:493
[Indexed for MEDLINE]

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