Detection of Cryptosporidium parvum in soil extracts

Mark Walker; Douglas Redelman

doi:10.1128/AEM.70.3.1827-1829.2004

Detection of Cryptosporidium parvum in soil extracts

Appl Environ Microbiol. 2004 Mar;70(3):1827-9. doi: 10.1128/AEM.70.3.1827-1829.2004.

Authors

Mark Walker¹, Douglas Redelman

Affiliation

¹ Department of Natural Resources and Environmental Sciences, University of Nevada, Reno, Nevada 89557, USA. mwalker@equinox.unr.edu

Abstract

Epifluorescent microscopy and flow cytometry were used in different combinations with fluorescein isothiocyanate-labeled immunoglobulins M and G3 to estimate the numbers of Cryptosporidium parvum oocysts in soil extracts containing 10 to 10,017 oocysts/ml. No combination had a systematic effect on accuracy or precision. Background debris may have produced overestimates at low oocyst concentrations when flow cytometry was used.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Animals
Antibodies, Protozoan
Cryptosporidium parvum / immunology
Cryptosporidium parvum / isolation & purification*
Flow Cytometry
Fluorescein-5-isothiocyanate
Fluorescent Dyes
Microscopy, Fluorescence
Oocysts / isolation & purification
Soil / parasitology*

Substances

Antibodies, Protozoan
Fluorescent Dyes
Soil
Fluorescein-5-isothiocyanate