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J Chromatogr. 1992 Apr 15;576(1):95-101.

Quantification of erythrocyte S-adenosyl-L-methionine levels and its application in enzyme studies.

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1
Department of Chemical Pathology, Faculty of Medicine, University of Pretoria, South Africa.

Abstract

A highly selective high-performance liquid chromatographic method for the quantification of human erythrocyte S-adenosyl-L-methionine levels is described. A strong cation-exchange sorbent with propylsulphonic acid functional groups was used to extract S-adenosyl-L-methionine and S-adenosylethionine (internal standard) from erythrocytes. Quantification of erythrocyte S-adenosyl-L-methionine levels was achieved by using reversed-phase high-performance liquid chromatography and ultraviolet detection at 254 nm. This method was adapted to measure methionine-adenosyltransferase activity in erythrocytes, which enables us to study the possible role of altered methylation in different diseases.

PMID:
1500462
[Indexed for MEDLINE]

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