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J Clin Microbiol. 2004 Mar;42(3):1290-3.

Real-time multiplex PCR assay for detection of Brucella spp., B. abortus, and B. melitensis.

Author information

1
Microbial Diseases Laboratory, Division of Communicable Disease Control, California Department of Health Services, Richmond, California 94804, USA. wprobert@dhs.ca.gov

Abstract

The identification of Brucella can be a time-consuming and labor-intensive process that places personnel at risk for laboratory-acquired infection. Here, we describe a real-time PCR assay for confirmation of presumptive Brucella isolates. The assay was designed in a multiplex format that will allow the rapid identification of Brucella spp., B. abortus, and B. melitensis in a single test.

PMID:
15004098
PMCID:
PMC356861
[Indexed for MEDLINE]
Free PMC Article

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