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J Microbiol Methods. 2004 Apr;57(1):9-16.

The evaluation of a mupirocin-based selective medium for the enumeration of bifidobacteria from probiotic animal feed.

Author information

1
Teagasc, Dairy Products Research Centre, Moorepark, Fermoy, Co. Cork, Ireland. pross@moorepark.teagasc.ie

Abstract

In this study, MRS medium supplemented with cysteine hydrochloride and mupirocin, termed Bifidobacterium selective medium (BSM) was found to be elective for bifidobacteria but inhibitory to a wide range of non-bifidobacteria strains commonly included in probiotic animal feed. Bacilli, lactobacilli, lactococci and streptococci failed to form colonies on BSM and enterococci, pediococci and propionibacteria formed colonies <0.5 mm in diameter. Bifidobacteria formed colonies >1 mm in size and could be readily distinguished. The addition of nystatin to BSM further inhibited Saccharomyces cerevisiae. BSM was successfully used to enumerate the bifidobacteria components, confirmed through fructose-6-phophate-phosphoketolase detection, present in two commercial probiotic feeds. The medium is recommended for the enumeration of bifidobacteria from animal feeds especially when not a numerically dominant component.

PMID:
15003683
DOI:
10.1016/j.mimet.2003.11.010
[Indexed for MEDLINE]

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