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Vaccine. 2004 Mar 12;22(9-10):1177-87.

Display of heterologous antigens on the Bacillus subtilis spore coat using CotC as a fusion partner.

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Dipartimento di Fisiologia Generale ed Ambientale, Sezione di Microbiologia, Università Federico II, Napoli, Italy.


We report the use of CotC, a major component of the Bacillus subtilis spore coat, as a fusion partner for the expression of two heterologous antigens on the spore coat. Recombinant spores expressing tetanus toxin fragment C (TTFC) of Clostridium tetani or the B subunit of the heat-labile toxin of Escherichia coli (LTB) were used for oral dosing and shown to generate specific systemic and mucosal immune responses in a murine model. This report, expanding the previously described expression of TTFC on the spore surface by fusion to CotB [J Bacteriol 183 (2001) 6294] and its use for oral vaccination [Infect Immun 71 (2003) 2810] shows that different antigens can be successfully presented on the spore coat and supports the use of the spore as an efficient vehicle for mucosal immunisation.

[Indexed for MEDLINE]

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