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FEBS Lett. 1992 Aug 17;308(2):161-4.

Characterisation of a human bilirubin UDP-glucuronosyltransferase stably expressed in hamster lung fibroblast cell cultures.

Author information

1
Department of Biochemical Medicine, University of Dundee, Ninewells Hospital and Medical School, Scotland, UK.

Abstract

A cDNA encoding a human bilirubin UDP-glucuronosyltransferase has been isolated and stably expressed in Chinese hamster V79 lung fibroblast cell line. Western blotting of cell homogenates with anti-UGT antibody revealed a highly expressed protein of approx. 55.5 kDa in size. The expressed enzyme specifically catalysed the formation of bilirubin mono- and diglucuronides, and also catalysed the glucuronidation of two phenolic compounds, which are good substrates for other human UGT isoenzymes, at low rates.

PMID:
1499725
DOI:
10.1016/0014-5793(92)81266-o
[Indexed for MEDLINE]
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