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J Bacteriol. 2004 Mar;186(5):1571-3.

Characterization of two kinases involved in thiamine pyrophosphate and pyridoxal phosphate biosynthesis in Bacillus subtilis: 4-amino-5-hydroxymethyl-2methylpyrimidine kinase and pyridoxal kinase.

Author information

1
Department of Chemistry and Chemical Biology, Cornell University, Ithaca, New York 14853, USA.

Abstract

Two Bacillus subtilis genes encoding two proteins (currently annotated ThiD and YjbV) were overexpressed and characterized. YjbV has 4-amino-5-hydroxymethyl-2-methylpyrimidine and 4-amino-5-hydroxymethyl-2-methylpyrimidine pyrophosphate kinase activity and should be reannotated ThiD, and B. subtilis ThiD has pyridoxine, pyridoxal, and pyridoxamine kinase activity and should be reannotated PdxK.

PMID:
14973012
PMCID:
PMC344394
DOI:
10.1128/jb.186.5.1571-1573.2004
[Indexed for MEDLINE]
Free PMC Article

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