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Biochim Biophys Acta. 2004 Feb 10;1661(1):97-105.

Identification of membrane-active regions of the HIV-1 envelope glycoprotein gp41 using a 15-mer gp41-peptide scan.

Author information

1
Instituto de Biología Molecular y Celular, Universidad Miguel Hernández, E-03202 Elche-Alicante, Spain.

Abstract

The identification of membrane-active regions of the ectodomain of the HIV-1 envelope glycoprotein gp41 has been made by determining the effect on membrane integrity of a 15-mer gp41-derived peptide library. By monitoring the effect of this peptide library on membrane leakage, we have identified three regions on the gp41 ectodomain with membrane-interacting capabilities: Region 1, which would roughly correspond to the polar sequence which follows the fusion domain and extends to the N-terminal heptad repeat region; Region 2, which would correspond to the immunodominant loop; and Region 3, which would correspond to the pre-transmembrane region of gp41. The identification of these three regions supports their direct role in membrane fusion as well as facilitating the future development of HIV-1 entry inhibitors.

PMID:
14967479
DOI:
10.1016/j.bbamem.2003.12.003
[Indexed for MEDLINE]
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