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J Microbiol Methods. 2004 Mar;56(3):427-30.

Purification of Toxoplasma gondii oocysts by cesium chloride gradient.

Author information

1
UPRES EA 3174, Neuroparasitologie et neuroépidémiologie tropicale, Faculté de Médecine, 2 Rue du Dr Marcland, F-87025 Limoges, France.

Abstract

We describe the use of a cesium chloride (CsCl) gradient as an improvement for the purification of Toxoplasma gondii oocysts from concentrated suspensions. After concentration by sucrose flotation, this technique gives a > 96% recovery of very pure unsporulated or sporulated oocysts, but requires "fresh" oocysts (< or = 10 weeks of age). This material is suitable for biochemical and immunological analyses of environmentally resistant T. gondii oocysts.

PMID:
14967234
DOI:
10.1016/j.mimet.2003.11.020
[Indexed for MEDLINE]

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