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Wei Sheng Yan Jiu. 2003 Nov;32(6):599-601.

[Study on protective action of lycium barbarum polysaccharides on DNA imparments of testicle cells in mice].

[Article in Chinese]

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School of Public Health, Wuhan University, Wuhan 430071, China.


To investigate the protective effect of lycium barbarum polysaccharides (LBP) on DNA oxidative damage of testicle cells induced by hydrogen peroxide (H2O2). The single cell gel electrophoresis(SCGE) was used to detect the breakage of DNA strand and analyze LBP protection against oxidation damage in testicle cells treated by different concentrations of LBP for 1 hour firstly, and then cultured with 100 mumol/L H2O2 for 25 min. The results showed that a certain moment action by H2O2 could induce the breakage of DNA strand. The pretreatment of LBP (50, 100, 200, 400 micrograms/ml) significantly decreased the frequencies of cells with tail moment and the tail length of testicle cells treated by H2O2. It is suggested that LBP itself could not cause the oxidation damage, but it could clean out the free-radical and restrain the DNA damage of testicle cells caused by the oxidative stress.

[Indexed for MEDLINE]

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