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J Chromatogr. 1992 Nov 6;582(1-2):49-55.

Quantitative analysis of collagen and elastin cross-links using a single-column system.

Author information

1
Department of Veterinary Medicine, University of Bristol, Langford, UK.

Abstract

The separation of both the immature and mature cross-links present in collagen together with the stable cross-links of elastin has been achieved on a single ion-exchange column. This technique avoids the current necessity for two different systems, ion-exchange and high-performance liquid chromatography-fluorescence techniques. The value of the method is illustrated by the comparison of the cross-link contents of aging bovine skin, from foetal to old age.

PMID:
1491057
DOI:
10.1016/0378-4347(92)80301-6
[Indexed for MEDLINE]

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