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FEMS Microbiol Lett. 1992 Dec 1;78(2-3):299-304.

The PurR binding site in the glyA promoter region of Escherichia coli.

Author information

1
Department of Microbiology, University of Iowa, Iowa City 52242.

Abstract

Site-directed mutagenesis was used to change the PurR binding site in the control region of a glyA-lac gene fusion. Mutations that changed the PurR binding sequence away from the consensus sequence reduced PurR binding, which correlated with reduced purine-mediated repression. Mutations that changed the binding sequence toward the consensus sequence had no significant effect on either PurR binding or purine-mediated repression. Hypoxanthine and guanine, co-repressors for PurR-mediated regulation of the pur regulon, increased binding of PurR to glyA operator DNA.

PMID:
1490614
DOI:
10.1016/0378-1097(92)90044-o
[Indexed for MEDLINE]

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