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Anal Biochem. 1992 Nov 15;207(1):186-92.

Fluorimetric DNA assay for cell growth estimation.

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1
Histopathology Unit, Imperial Cancer Research Fund, London, United Kingdom.

Abstract

A growth assay using the fluorescent dye Hoechst 33258 has been developed which allows sensitive and rapid analysis of the DNA content of a variety of cell types including keratinocytes and mucus-secreting cells, and which requires a minimum of liquid handling. The assay can detect as few as 500 diploid human cells, and is compatible with the simultaneous detection of [3H]thymidine incorporation in the same cultures.

PMID:
1489093
[Indexed for MEDLINE]
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