Format

Send to

Choose Destination
Arch Microbiol. 1992;158(6):412-7.

Maleylacetate reductase of Pseudomonas sp. strain B13: dechlorination of chloromaleylacetates, metabolites in the degradation of chloroaromatic compounds.

Author information

1
Chemische Mikrobiologie der Bergischen Universit├Ąt-Gesamthochschule Wuppertal, Federal Republic of Germany.

Abstract

The maleylacetate reductase of 3-chlorobenzoate-grown cells of Pseudomonas sp. strain B13 has been purified 50-fold. The enzyme converted 2-chloromaleylacetate to 3-oxoadipate with temporary occurrence of maleylacetate; 1 mol of chloride was eliminated during the conversion of 1 mol of 2-chloro- and 2,3-dichloromaleylacetate; 2 mol of NADH were consumed per mol of 2-chloro- and 2,3-dichloromaleylacetate while only 1 mol was necessary to catalyze the conversion of maleylacetate or 2-methylmaleylacetate. The maleylacetate reductase failed to use fumarylacetate as a substrate. The role of the enzyme in the chloroaromatics degradation is discussed.

PMID:
1482270
DOI:
10.1007/bf00276301
[Indexed for MEDLINE]

Supplemental Content

Loading ...
Support Center