Characterization of polysomes and polysomal mRNAs by sucrose density gradient centrifugation followed by immobilization in polyacrylamide gel matrix

Methods Enzymol. 1992:216:168-79. doi: 10.1016/0076-6879(92)16020-k.

Authors

A D Bharucha¹, M R Ven Murthy

Affiliation

¹ Department of Biochemistry, Faculty of Medicine, Laval University, Ste-Foy, Quebec, Canada.

PMID: 1479900
DOI: 10.1016/0076-6879(92)16020-k

No abstract available

MeSH terms

Acrylamide
Acrylamides
Animals
Brain / ultrastructure*
Brain Chemistry*
Cell Fractionation / methods
Centrifugation, Density Gradient / instrumentation
Centrifugation, Density Gradient / methods
Gels
Indicators and Reagents
Polyribosomes / ultrastructure*
RNA, Messenger / isolation & purification*
Rats
Spectrophotometry / methods

Substances

Acrylamides
Gels
Indicators and Reagents
RNA, Messenger
Acrylamide