Studies on thermophile products. V. Immunosuppressive profile in vitro of Bacillus stearothermophilus component, Fr.5-B

Chem Pharm Bull (Tokyo). 1992 Nov;40(11):3017-20. doi: 10.1248/cpb.40.3017.

Abstract

The immunosuppressive profile of Bacillus stearothermophilus UK563 component, Fr.5-B, is presented in in vitro studies. Fr.5-B (0.1-1000 ng/ml), provided it was added at the initiation of mixed leukocyte reaction (MLR), inhibited dose-dependently the incorporation of tritiated thymidine ([3H]TdR) into mouse spleen cells and human peripheral blood lymphocytes. Even the addition of Fr.5-B 48 h after the onset of culture suppressed mouse MLR, unlike cyclosporin A (CYA). Fr.5-B significantly inhibited cytotoxic T lymphocyte generation determined by [3H]TdR-release micro-cytotoxicity assay by using mouse mastocytoma P815 as targets. Moreover, this component decreased dose-dependently the expression of class II major histocompatibility molecules (Ia) on mouse peritoneal macrophages induced by concanavalin A supernatant. The present results revealed the unique immunosuppressive property of Fr.5-B which was different from that of CYA.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Cells, Cultured
  • Cyclosporine / pharmacology
  • Cytotoxicity, Immunologic / drug effects
  • Female
  • Geobacillus stearothermophilus / chemistry*
  • Histocompatibility Antigens Class II / analysis
  • Humans
  • Immune Tolerance*
  • Immunosuppressive Agents / immunology*
  • Immunosuppressive Agents / pharmacology*
  • Lymphocyte Culture Test, Mixed
  • Macrophages / drug effects
  • Macrophages / immunology
  • Mice
  • Organic Chemicals
  • T-Lymphocytes, Cytotoxic / drug effects
  • T-Lymphocytes, Cytotoxic / immunology
  • Tumor Cells, Cultured

Substances

  • Fr.5-B, Bacillus stearothermophilus UK563 component
  • Histocompatibility Antigens Class II
  • Immunosuppressive Agents
  • Organic Chemicals
  • Cyclosporine