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PCR Methods Appl. 1992 Nov;2(2):144-8.

A lock-docking oligo(dT) primer for 5' and 3' RACE PCR.

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1
Department of Biochemistry and Molecular Biology, School of Medicine, University of Minnesota, Duluth 55812.

Abstract

We describe a method that can be used to obtain and sequence 3' and 5' ends of cDNA transcripts directly from PCR products. The method employs a modified oligo(dT) primer that enables it to "lock-dock" at the junction of gene-specific cDNA sequence and a natural (3') or appended (5') poly(A) tail. As a result, discrete, first-round PCR products are obtained that are easily isolated and sequenced directly.

PMID:
1477669
[Indexed for MEDLINE]
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