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Methods Mol Biol. 2004;257:171-80.

A poly(A) tail-responsive in vitro system for cap- or IRES-driven translation from HeLa cells.

Author information

1
Gene Expression Programme, EMBL, Heidelberg, Germany.

Abstract

In cells, the poly(A) tail stimulates translation from messenger RNAs bearing a cap structure or viral IRES elements. This 3' end-mediated stimulation of translation is not reflected in commonly used commercial cell-free translation systems prepared from rabbit reticulocytes or wheat germ. We describe a simple procedure to generate poly(A) tail-responsive translation extracts from HeLa cells. We suggest that this procedure should be adaptable to many animal cell lines.

PMID:
14770005
DOI:
10.1385/1-59259-750-5:171
[Indexed for MEDLINE]

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