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[Study on the relationship of MTHFR polymorphisms with unexplained recurrent spontaneous abortion].

[Article in Chinese]

Author information

1
Department of Obstetric and Gynecology, Qilu Hospital, Shandong University, Jinan, Shandong, PR China. lixmei@sina.com

Abstract

OBJECTIVE:

To assess the relationship of methylenetetrahydrofolate reductase (MTHFR) C677T genotypes to unexplained recurrent spontaneous abortion (URSA).

METHODS:

This study included two groups:57 currently non-pregnant women with a history of URSA (URSA group), and 50 currently non-pregnant women with a history of having given birth to at least one live baby and without any history of spontaneous abortion, still-born fetus, placental thrombosis and intrauterine growth retardation(IUGR)(control group). The fasting serum-Hcy was measured with high pressure liquid chromatography. Folic acid and vitamin B(12) were detected by radioimmune assay; antiphospholipid antibody (ACA) was detected by ELISA. MTHFR C677T gene polymorphisms were detected by the technique of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).

RESULTS:

C/C genotype in URSA group was significantly lower than that in control group, the total mutant T allele frequency was significantly higher than that in control group. There was no significant difference in respect of "age, rural area/city, period, primary/secondary abortion" between the genotype distributions of MTHFR C677T. The T/T genotype and C/T+T/T genotypes frequencies for "abortion times>or=3" were higher than those for "abortion time <3".

CONCLUSION:

MTHFR C677T gene polymorphism is a genetic risk factor for URSA.

PMID:
14767907
[Indexed for MEDLINE]

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