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Biotechniques. 1992 Dec;13(6):901-5.

Rapid identification of high affinity peptide ligands using positional scanning synthetic peptide combinatorial libraries.

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Torrey Pines Institute for Molecular Studies, San Diego, CA 92121.


We describe here a conceptually unique set of individual synthetic peptide combinatorial libraries (SPCLs), termed a positional scanning SPCL (PS-SPCL), that can be used for the rapid (i.e., a single day) identification of peptide sequences that bind with high affinity to antibodies, receptors or other acceptor molecules. The PS-SPCL described here is made up of six individual positional peptide libraries, each one consisting of hexamers with a single position defined and five positions as mixtures. As an example of the utility of such PS-SPCLs, the antigenic determinants recognized by two different monoclonal antibodies were correctly identified upon a single screening.

[Indexed for MEDLINE]

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