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FEBS Lett. 2004 Jan 30;558(1-3):63-8.

Conjugate for efficient delivery of short interfering RNA (siRNA) into mammalian cells.

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1
MRC-Dunn Human Nutrition Unit, Wellcome Trust/MRC building, Hills Rd, Cambridge CB2 2XY, UK.

Erratum in

  • FEBS Lett. 2004 May 21;566(1-3):317.

Abstract

The efficient delivery of short interfering RNAs (siRNAs) into cells provides a powerful approach to study cellular functions. SiRNAs were coupled to the membrane permeant peptides (MPPs) penetratin and transportan to improve their uptake by cells. Thiol-containing siRNAs corresponding to luciferase, or green fluorescent protein (GFP) transgenes, were synthesized and conjugated to penetratin or transportan via a disulfide bond that is labile in the reducing environment of the cytoplasm. These MPP-siRNAs efficiently reduced transient and stable expression of reporter transgenes in several mammalian cell types in a high proportion of cells, and demonstrated equivalent or better delivery characteristics than cationic liposomes with fewer manipulations.

PMID:
14759517
DOI:
10.1016/S0014-5793(03)01505-9
[Indexed for MEDLINE]
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