Preparative isolation and purification of isoflavan and pterocarpan glycosides from Astragalus membranaceus Bge. var. mongholicus (Bge.) Hsiao by high-speed counter-current chromatography

Xiaofeng Ma; Pengfei Tu; Yingjie Chen; Tianyou Zhang; Yun Wei; Yoichiro Ito

doi:10.1016/j.chroma.2003.10.014

Preparative isolation and purification of isoflavan and pterocarpan glycosides from Astragalus membranaceus Bge. var. mongholicus (Bge.) Hsiao by high-speed counter-current chromatography

J Chromatogr A. 2004 Jan 16;1023(2):311-5. doi: 10.1016/j.chroma.2003.10.014.

Authors

Xiaofeng Ma¹, Pengfei Tu, Yingjie Chen, Tianyou Zhang, Yun Wei, Yoichiro Ito

Affiliation

¹ Department of Biology, Graduate School of Chinese Academy of Science, P.O. Box 3908, Beijing 100039, China.

PMID: 14753698
DOI: 10.1016/j.chroma.2003.10.014

Abstract

(3R)-(-)-7,2'-Dihydroxy-3',4'-dimethyl isoflavan-7-O-beta-D-glucopyranoside and (6aR, 11aR) 9,10-di-methoxypterocarpan-3-O-beta-D-glucopyranoside were separated from the ethyl acetate extract of the root of Astragalus membranaceus Bge. var. mongholicus (Bge.) Hsiao by high-speed counter-current chromatography (HSCCC). A two-phase system composed of ethyl acetate-ethanol-acetic acid-water (4:1:0.25:5, v/v) was selected by analytical HSCCC. Preparative HSCCC yielded, from 100 mg of the partially purified extract, 50 mg of isoflavan glycoside and 10 mg of pterocarpan glycoside each at over 95% purity by high-performance liquid chromatography (HPLC) analysis. Their structures were identified by MS, 1H NMR and 13C NMR.

MeSH terms

Astragalus Plant / chemistry*
Chromatography, High Pressure Liquid
Countercurrent Distribution / methods*
Glycosides / chemistry*
Magnetic Resonance Spectroscopy
Mass Spectrometry

Substances

Glycosides