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Biotechnol Bioeng. 2004 Feb 5;85(3):277-82.

Poly-3-hexyl thiophene Langmuir-Blodgett films for application to glucose biosensor.

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Biomolecular Electronics & Conducting Polymer Research Group, National Physical Laboratory, Dr. KS Krishnan Road, New Delhi- 110012, India.


Langmuir-Blodgett films of poly(3-hexyl thiophene) have been prepared by simultaneous entrapment of glucose oxidase and transferred onto the indium-tin-oxide coated glass plates. The films have been characterized by FTIR spectroscopy and detailed response studies have been performed with respect to glucose concentration, temperature, and storage time. These P3HT/SA/GOX electrodes have been utilized for glucose estimation from 100-500 mg/dL by amperometric method. The electrodes have been found to have sensitivity of 0.75 nA/mg/dL detection limit of 50 mg/dL and shelf life of about 75 days at 4 degrees C.

[Indexed for MEDLINE]

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