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C R Biol. 2003 Oct-Nov;326(10-11):967-70.

LNCIB human full-length cDNAs collection: towards a better comprehension of the human transcriptome.

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Laboratorio Nazionale CIB, Functional Genomics Group, AREA Science Park, Padriciano 99, Trieste 34012, Italy.


LNCIB has been producing a variety of human full-length-enriched, normalized and subtracted cDNA libraries from various cell lines and tissues in different developmental stages by using the CAP-Trapper method. By sequencing 23000 clones of these libraries we identified a pool of about 5800 good quality unique cDNAs. After BLAST analysis on Human RefSeq/Unigene databases, 1717 of these sequences remained with no or poor annotation. We show that cross-species comparative BLAST resulted as a valid tool for the annotation of orthologous genes.

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