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J Am Chem Soc. 2004 Jan 28;126(3):739-43.

Control of protein structure and function through surface recognition by tailored nanoparticle scaffolds.

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  • 1Department of Chemistry, University of Massachusetts, Amherst, Massachusetts 01003, USA.

Abstract

Thioalkyl and thioalkylated oligo(ethylene glycol) (OEG) ligands with chain-end functionality were used to fabricate water-soluble CdSe nanoparticle scaffolds. Surface recognition of chymotrypsin (ChT) was achieved using these functionalized nanoparticle scaffolds, with three levels of interaction demonstrated: no interaction (OEG terminated with hydroxyl group), inhibition with denaturation (carboxylate-terminated thioalkyl ligands), and inhibition with retention of structure (carboxylate-terminated OEG). The latter process was reversible upon an increase in ionic strength, with essentially complete restoration of enzymatic activity.

PMID:
14733547
DOI:
10.1021/ja037470o
[PubMed - indexed for MEDLINE]
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