Send to

Choose Destination
Biochem Biophys Res Commun. 1992 Dec 15;189(2):1008-16.

Targeting aequorin to the endoplasmic reticulum of living cells.

Author information

Department of Medical Biochemistry, University of Wales College of Medicine, Heath Park, Cardiff, UK.


The photoprotein aequorin has been engineered with an ER targeting sequence at the N-terminus, with and without KDEL at the C-terminus, so that it locates in the ER-secretory pathway. For the first time the free Ca2+ has been quantified inside the ER and shown to be 5-20 times that in the cytosol. In COS cells free Ca2+ in the ER ranged from 1-5mM at 37 degrees C, decreasing 2-5-fold within 1 min of exposure to the Ca2+ ionophore ionomycin in the absence of external Ca2+.

[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center