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Biochem Biophys Res Commun. 1992 Dec 15;189(2):1008-16.

Targeting aequorin to the endoplasmic reticulum of living cells.

Author information

1
Department of Medical Biochemistry, University of Wales College of Medicine, Heath Park, Cardiff, UK.

Abstract

The photoprotein aequorin has been engineered with an ER targeting sequence at the N-terminus, with and without KDEL at the C-terminus, so that it locates in the ER-secretory pathway. For the first time the free Ca2+ has been quantified inside the ER and shown to be 5-20 times that in the cytosol. In COS cells free Ca2+ in the ER ranged from 1-5mM at 37 degrees C, decreasing 2-5-fold within 1 min of exposure to the Ca2+ ionophore ionomycin in the absence of external Ca2+.

PMID:
1472014
DOI:
10.1016/0006-291x(92)92304-g
[Indexed for MEDLINE]

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