Cloning and sequence analysis of the Candida utilis HIS3 gene

Liliana Basabe; Iveldris Domínguez; Francisco P Chávez

doi:10.1016/s1389-0344(02)00114-4

Cloning and sequence analysis of the Candida utilis HIS3 gene

Biomol Eng. 2004 Jan;21(1):15-20. doi: 10.1016/s1389-0344(02)00114-4.

Authors

Liliana Basabe¹, Iveldris Domínguez, Francisco P Chávez

Affiliation

¹ Center for Genetic Engineering and Biotechnology, Agriculture Division, 31 Ave /158 & 190, PO Box 6162, Havana 10600, Cuba. liliana.basabe@cigb.edu.cu

PMID: 14715315
DOI: 10.1016/s1389-0344(02)00114-4

Abstract

A DNA fragment, carrying the Candida utilis HIS3 gene, has been isolated from a genomic DNA library by complementation of the E. coli hisB mutant. Its nucleotide sequence was determined and it predicts a single open reading frame of 675 bp (224 aa). The deduced amino acid sequence is highly homologous to other yeast and fungi HIS3 genes.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Candida / enzymology*
Candida / genetics*
Cloning, Molecular
Gene Expression Regulation, Enzymologic / genetics
Gene Expression Regulation, Fungal / genetics
Hydro-Lyases / chemistry*
Hydro-Lyases / genetics*
Hydro-Lyases / metabolism
Molecular Sequence Data
Open Reading Frames / genetics
Sequence Homology, Amino Acid

Substances

Hydro-Lyases
imidazoleglycerolphosphate dehydratase