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Anal Biochem. 2004 Feb 1;325(1):68-76.

Use of protein biotinylation in vivo for chromatin immunoprecipitation.

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  • 1Laboratoire Oncogénse, Différenciation et Transduction du Signal, Institut André Lwoff, 7 rue Guy Moquet, 94800 Villejuif, France.

Abstract

We describe a system designed to express biotinylated proteins in mammalian cells in vivo and its application to the study of protein-DNA interactions in vivo by chromatin immunoprecipitation (ChIP). The system is based on coexpression of the target protein fused to a short biotin acceptor domain together with the biotinylating enzyme BirA from Escherichia coli. The superior strength of the biotin-avidin interaction allows one to employ more stringent washing conditions in the ChIP protocol, resulting in a better signal/noise ratio.

PMID:
14715286
[PubMed - indexed for MEDLINE]
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