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Fertil Steril. 2004 Jan;81(1):73-9.

Comparison of mitochondrial DNA contents in human embryos with good or poor morphology at the 8-cell stage.

Author information

1
Institute of Biochemistry, Chung Shan Medical University, ROC, Taichung, Taiwan.

Abstract

OBJECTIVE:

To quantify the mitochondrial DNA contents in human embryos with good or poor quality at the 8-cell stage.

DESIGN:

Prospective study.

SETTING:

Private infertility clinic.

PATIENT(S):

Five women aged 24 to 34 years.

INTERVENTION(S):

Embryos obtained in standard superovulation and embryo culture procedures.

MAIN OUTCOME MEASURE(S):

Mitochondrial DNA (mtDNA) copy numbers in human embryos at cleavage stage were quantified by real-time polymerase chain reaction, in an effort to correlate with morphology.

RESULT(S):

The grade 8A embryos contained a mean mtDNA copy number at 1163937 (n = 8, from three patients); grade 8B embryos, at 939345 (n = 5, from two patients); grade 8C(+) embryos, at 637872 (n = 12, from 5 patients); and grade 8C(+) embryos derived from 3PN zygotes, at 300429 (n = 3, from a single patient).

CONCLUSION(S):

Great variations were found among blastomeres from a single embryo and among embryos from a single patient. The native variations of mtDNA copy number may affect developmental ability irrespective of morphology.

[Indexed for MEDLINE]

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