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FEBS Lett. 2004 Jan 2;556(1-3):47-52.

Disruption of gamma-glutamylcysteine synthetase results in absolute glutathione auxotrophy and apoptosis in Candida albicans.

Author information

1
Laboratory of Biophysics, School of Biological Sciences, and Institute of Microbiology, Seoul National University, 151-742, Seoul, South Korea.

Abstract

Glutathione is the most abundant non-protein thiol and a major source of reducing equivalents in eukaryotes. We examined the role of glutathione in Candida albicans by the disruption of gamma-glutamylcysteine synthetase (GCS1), an essential enzyme in glutathione biosynthesis. The gcs1/gcs1 null mutants exhibited glutathione auxotrophy, which could be rescued by supplementing with reduced and oxidized glutathione and gamma-glutamylcysteine. When the mutants were depleted of glutathione, they showed typical markers of apoptosis. These results suggest that glutathione itself is an essential metabolite and C. albicans lacking GCS1 undergoes apoptosis.

PMID:
14706824
DOI:
10.1016/s0014-5793(03)01363-2
[Indexed for MEDLINE]
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