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J Microbiol Methods. 2004 Jan;56(1):119-24.

High phosphate (up to 600 mM) induces pseudohyphal development in five wild type Candida albicans.

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School of Biological Sciences, University of Nebraska, Lincoln, NE 68588-0666, USA.


A method is described for the formation of nearly 100% pseudohyphae populations of wild-type Candida albicans A72. The method employs fungal growth at 37 degrees C (ca. 5x10(6) cells/ml) in a glucose-proline-N-acetyl-glucosamine medium supplemented with up to 600 mM phosphate (KH(2)PO(4)/K(2)HPO(4) 1:1) at pH 6.5. Four other strains of C. albicans (MEN, 10261, SG5314 and CAI-4) also formed pseudohyphae under these conditions, although the phosphate response profiles differed in the concentration required for each strain to form pseudohyphae.

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