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BMC Genomics. 2004 Jan 5;5(1):1.

SAGE is far more sensitive than EST for detecting low-abundance transcripts.

Author information

1
Department of Medicine, University of Chicago, 5841 S, Maryland Avenue, MC2115, Chicago, Illinois 60637, USA. msun@medicine.bsd.uchicago.edu

Abstract

BACKGROUND:

Isolation of low-abundance transcripts expressed in a genome remains a serious challenge in transcriptome studies. The sensitivity of the methods used for analysis has a direct impact on the efficiency of the detection. We compared the EST method and the SAGE method to determine which one is more sensitive and to what extent the sensitivity is great for the detection of low-abundance transcripts.

RESULTS:

Using the same low-abundance transcripts detected by both methods as the targeted sequences, we observed that the SAGE method is 26 times more sensitive than the EST method for the detection of low-abundance transcripts.

CONCLUSIONS:

The SAGE method is more efficient than the EST method in detecting the low-abundance transcripts.

PMID:
14704093
PMCID:
PMC317289
DOI:
10.1186/1471-2164-5-1
[Indexed for MEDLINE]
Free PMC Article

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