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J Electron Microsc (Tokyo). 2003;52(5):465-9.

Effect of surface lipopolysaccharide on the nature of membrane vesicles liberated from the Gram-negative bacterium Pseudomonas aeruginosa.

Author information

1
Department of Microbiology and Canadian Bacterial Disease Network-NCE University of Guelph, Guelph, Ontario, Canada N1G 2W1.

Abstract

Membrane vesicles (MVs) were isolated from three isogenic lipopolysaccharide (LPS) mutants derived from the Gram-negative bacterium Pseudomonas aeruginosa PAO1 and compared with the parent strain. Parent cells possessed serotype 05 (A+B+) LPS whereas the three mutants contained A+B-, A-B+ and A-B-. The MVs from the mutants contained their expected phenotypic LPS and varied in size, especially A-B- MVs. Mass and total protein differences were also noted. When the parent and mutant cells were treated with the outer membrane-perturbing antibiotic gentamicin, all cells produced 3-5 times more MVs and these had great variation in size, mass and total protein. It is concluded that the type of cellular LPS affects both the natural and gentamicin-induced development of MVs.

PMID:
14700078
DOI:
10.1093/jmicro/52.5.465
[Indexed for MEDLINE]

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