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Genetics. 2003 Nov;165(3):1433-41.

Green fluorescent protein tagging Drosophila proteins at their native genomic loci with small P elements.

Author information

1
Department of Biochemistry and Biophysics, University of California, San Francisco, California 94143-0448, USA.

Erratum in

  • Genetics. 2004 Aug;167(4):2143.

Abstract

We describe a technique to tag Drosophila proteins with GFP at their native genomic loci. This technique uses a new, small P transposable element (the Wee-P) that is composed primarily of the green fluorescent protein (GFP) sequence flanked by consensus splice acceptor and splice donor sequences. We demonstrate that insertion of the Wee-P can generate GFP fusions with native proteins. We further demonstrate that GFP-tagged proteins have correct subcellular localization and can be expressed at near-normal levels. We have used the Wee-P to tag genes with a wide variety of functions, including transmembrane proteins. A genetic analysis of 12 representative fusion lines demonstrates that loss-of-function phenotypes are not caused by the Wee-P insertion. This technology allows the generation of GFP-tagged reagents on a genome-wide scale with diverse potential applications.

PMID:
14668392
PMCID:
PMC1462835
[Indexed for MEDLINE]
Free PMC Article

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