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Plant Mol Biol. 1992 Dec;20(6):1195-7.

New plant binary vectors with selectable markers located proximal to the left T-DNA border.

Author information

1
Max-Planck-Institut für Züchtungsforschung, Köln, Germany.

Abstract

Five new binary vectors have been constructed which have the following features: (1) different plant selectable markers including neomycin phosphotransferase (nptII), hygromycin phosphotransferase (hpt), dihydrofolate reductase (dhfr), phosphinothricin acetyl transferase (bar), and bleomycin resistance (ble); (2) selectable markers are located near the T-DNA left border and; (3) selectable marker and beta-glucuronidase (uidA) reporter genes are divergently organized for efficient expression, and can easily be removed or replaced as needed.

PMID:
1463855
[Indexed for MEDLINE]

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