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J Pharm Biomed Anal. 2003 Nov 24;33(4):539-44.

Determination of hamamelitannin, catechins and gallic acid in witch hazel bark, twig and leaf by HPLC.

Author information

1
R&D Department, William Ransom & Son plc, Hitchin, Herts SG5 1LY, UK. hwang@williamransom.com

Abstract

An HPLC method for the determination of hamamelitannin, catechins and gallic acid in witch hazel bark, twig and leaf has been developed. The separation system consisted of a C18 reversed-phase column, a gradient elution system of methanol/water and orthophosphoric acid, and a photodiode array detector. The concentrations of hamamelitannin, gallic acid, (+)-gallocatechin and (+)-catechin were 4.77, 0.59, 0.22 and 0.39% (w/w), respectively, in the bark. Hamamelitannin and catechins were also detected in the leaves at concentration less than 0.04% (w/w). This method is simple, sensitive and reproducible, ideally suited for rapid, routine analysis.

PMID:
14623578
DOI:
10.1016/s0731-7085(03)00303-0
[Indexed for MEDLINE]

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