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Plasmid. 1992 Nov;28(3):258-61.

Promoter probe and shuttle plasmids for Deinococcus radiodurans.

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Department of Pathology, F. E. H├ębert School of Medicine, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814-4799.


Two improved Deinococcus radiodurans-Escherichia coli shuttle vectors have been constructed. pI3 is a 16-kb plasmid that confers chloramphenicol resistance in D. radiodurans (CmR, cat) and ampicillin resistance in E. coli (ApR) and contains a multiple cloning site that does not interrupt sequences necessary for replication or drug resistance in either host. pI304 is a promoter-probe plasmid that is similar to pI3, but lacks the D. radiodurans promoting sequence for the cat gene, while retaining sequences necessary for replication.

[Indexed for MEDLINE]

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