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Electrophoresis. 2003 Nov;24(21):3769-77.

A massively parallel PicoTiterPlate based platform for discrete picoliter-scale polymerase chain reactions.

Author information

1
454 Life Sciences, Branford, CT 06405, USA. jleamon@454.com

Erratum in

  • Electrophoresis. 2004 Apr;25(7-8):1176.

Abstract

We demonstrate successful, simultaneous polymerase chain reaction (PCR) amplification of up to 300 000 discrete reactions in a novel platform, the PicoTiterPlate. In addition to elevated throughput, the PicoTiterPlate based amplifications (PTPCR) can be performed in extremely small volumes: individual reactions volumes are as low as 39.5 pL, with a total 15.3 microL reaction volume for the entire PicoTiterPlate. The bulk PTPCR product can be recovered and assayed with real-time PCR, or discrete PTPCR products can be driven to solid supports, enabling downstream applications such as translation/transcription or sequencing.

PMID:
14613204
DOI:
10.1002/elps.200305646
[Indexed for MEDLINE]

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