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J Exp Med. 2003 Nov 17;198(10):1453-62. Epub 2003 Nov 10.

LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling.

Author information

1
Institute of Molecular Genetics, AS CR, Vídenská 1083, 142 20 Praha 4, Czech Republic.

Abstract

Lymphocyte membrane rafts contain molecules critical for immunoreceptor signaling. Here, we report identification of a new raft-associated adaptor protein LIME (Lck-interacting molecule) expressed predominantly in T lymphocytes. LIME becomes tyrosine phosphorylated after cross-linking of the CD4 or CD8 coreceptors. Phospho-LIME associates with the Src family kinase Lck and its negative regulator, Csk. Ectopic expression of LIME in Jurkat T cells results in an increase of Csk in lipid rafts, increased phosphorylation of Lck and higher Ca2+ response to CD3 stimulation. Thus, LIME appears to be involved in regulation of T cell activation by coreceptors.

PMID:
14610046
PMCID:
PMC2194115
DOI:
10.1084/jem.20031484
[Indexed for MEDLINE]
Free PMC Article

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