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Science. 2003 Dec 19;302(5653):2124-2126. doi: 10.1126/science.1088697. Epub 2003 Nov 6.

Yeast life-span extension by calorie restriction is independent of NAD fluctuation.

Author information

1
Department of Pathology, Harvard Medical School, 200 Longwood Avenue, Boston MA 02115, USA.
2
Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Rua da Quinta Grande 6, Apartado 127, 2780-156 OEIRAS, Portugal.
3
Biomol Research Laboratories, Inc., 5120 Butler Pike, Plymouth Meeting, PA 19462, USA.
#
Contributed equally

Abstract

Calorie restriction (CR) slows aging in numerous species. In the yeast Saccharomyces cerevisiae, this effect requires Sir2, a conserved NAD+-dependent deacetylase. We report that CR reduces nuclear NAD+ levels in vivo. Moreover, the activity of Sir2 and its human homologue SIRT1 are not affected by physiological alterations in the NAD+:NADH ratio. These data implicate alternate mechanisms of Sir2 regulation by CR.

PMID:
14605207
PMCID:
PMC4998187
DOI:
10.1126/science.1088697
[Indexed for MEDLINE]
Free PMC Article

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