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J Clin Microbiol. 2003 Nov;41(11):5224-6.

Evaluation of genotyping large numbers of Escherichia coli isolates by enterobacterial repetitive intergenic consensus-PCR.

Author information

1
Department of Epidemiology, School of Public Health, University of Michigan, Ann Arbor, Michigan 48109, USA.

Abstract

We analyzed a large collection of Escherichia coli isolates typed by enterobacterial repetitive intergenic consensus (ERIC)-PCR with BioNumerics gel analysis software. However, the interexperimental variation of ERIC-PCR caused the computer software to classify repeated isolates as different. ERIC-PCR should not be used as the sole determinant of genetic similarity when typing large numbers of bacterial isolates via computer software.

PMID:
14605168
PMCID:
PMC262489
DOI:
10.1128/jcm.41.11.5224-5226.2003
[Indexed for MEDLINE]
Free PMC Article

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