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Arch Med Res. 2003 Sep-Oct;34(5):362-6.

Cross-reactivity between caprine arthritis-encephalitis virus and type 1 human immunodeficiency virus.

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1
Unidad de Investigación Médica en Inmunología, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Mexico City, DF, Mexico.

Abstract

BACKGROUND:

Caprine arthritis encephalitis (CAE) is caused by the lentivirus caprine arthritis-encephalitis virus (CAEV), a member of the Retroviridae family that also includes the human immunodeficiency virus (HIV). Serum of CAEV-infected goats cross-reacts with HIV-1 antigens in enzyme-linked immunosorbent assay (ELISA) tests. We attempted to identify the proteins responsible for this cross-reactivity.

METHODS:

Fifty selected human sera (30 positive, 10 negative, and 10 indeterminate to HIV-1 by Western blot) and 50 selected goat sera (33 positive and 17 negative to CAEV by ELISA) were evaluated. Human and goat sera were tested by Western blot against HIV-1 and CAEV antigens.

RESULTS:

Cross-reactivity between surface glycoproteins gp120 (HIV-1) and gp135 (CAEV) was specific. Positive reaction of human sera to CAEV gp135 was more intense than that of goat sera to HIV-1 gp120. Surface glycoprotein sequences of the two viruses were compared by Lasergene software (Dynex Technologies, Inc., Chantilly, VA, USA). Three homologous regions were identified: the first in the internal domain of gp120; the second in the beta3 loop, and still another-with the greatest homology-in a short sequence of the proximal region of the external domain of gp120 between loops beta4 and beta8.

CONCLUSIONS:

Surface glycoproteins of HIV-1 and CAEV share structural regions essential for viral adsorption and for induction of neutralizing antibodies. Thus, human contact with CAEV eventually could be a possible source of HIV-1 false positive reactions and must be considered in the interpretation of HIV serologic results.

PMID:
14602501
DOI:
10.1016/j.arcmed.2003.08.001
[Indexed for MEDLINE]
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