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Lancet. 2003 Nov 1;362(9394):1459-60.

Use of dried whole blood spots to measure CD4+ lymphocyte counts in HIV-1-infected patients.

Author information

1
UNZA-UCLMS (University of Zambia-University College London Medical School) Research and Training Project, University Teaching Hospital, School of Medicine, Box 50110, Lusaka, Zambia.

Abstract

As antiretroviral drugs become widely available in developing countries, practical, field-friendly, and cheap methods of measuring CD4+ lymphocyte counts need to be developed. We tested use of whole blood spots dried on filter paper to measure CD4+ lymphocyte counts. We obtained blood from 42 HIV-1-infected patients from Zambia. We dried blood spots on filter paper and measured CD4+ lymphocyte counts with an established commercial enzyme immunoassay. We compared these measurements with those obtained from matched liquid whole-blood samples analysed with standard flow cytometry. Results of the filter-paper method accorded well with flow cytometry CD4 counts greater than 200 cells/microL (mean difference 13.6 [SD 52.4]). Dried whole blood stored on filter paper could be developed into a field-friendly alternative for CD4+ lymphocyte count measurements.

PMID:
14602443
DOI:
10.1016/S0140-6736(03)14693-4
[Indexed for MEDLINE]

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