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Lancet. 2003 Nov 1;362(9394):1459-60.

Use of dried whole blood spots to measure CD4+ lymphocyte counts in HIV-1-infected patients.

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UNZA-UCLMS (University of Zambia-University College London Medical School) Research and Training Project, University Teaching Hospital, School of Medicine, Box 50110, Lusaka, Zambia.


As antiretroviral drugs become widely available in developing countries, practical, field-friendly, and cheap methods of measuring CD4+ lymphocyte counts need to be developed. We tested use of whole blood spots dried on filter paper to measure CD4+ lymphocyte counts. We obtained blood from 42 HIV-1-infected patients from Zambia. We dried blood spots on filter paper and measured CD4+ lymphocyte counts with an established commercial enzyme immunoassay. We compared these measurements with those obtained from matched liquid whole-blood samples analysed with standard flow cytometry. Results of the filter-paper method accorded well with flow cytometry CD4 counts greater than 200 cells/microL (mean difference 13.6 [SD 52.4]). Dried whole blood stored on filter paper could be developed into a field-friendly alternative for CD4+ lymphocyte count measurements.

[Indexed for MEDLINE]

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