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J Cereb Blood Flow Metab. 2003 Nov;23(11):1298-306.

Lactate is a preferential oxidative energy substrate over glucose for neurons in culture.

Author information

1
Centre de Résonance Magnétique des Systèmes Biologiques, UMR 5536 CNRS/Université, Bordeaux, France. akb@rmsb.ubordeaux2.fr

Erratum in

  • J Cereb Blood Flow Metab. 2004 Feb;24(2):270.

Abstract

The authors investigated concomitant lactate and glucose metabolism in primary neuronal cultures using 13C- and 1H-NMR spectroscopy. Neurons were incubated in a medium containing either [1-13C]glucose and different unlabeled lactate concentrations, or unlabeled glucose and different [3-13C]lactate concentrations. Overall, 13C-NMR spectra of cellular extracts showed that more 13C was incorporated into glutamate when lactate was the enriched substrate. Glutamate 13C-enrichment was also found to be much higher in lactate-labeled than in glucose-labeled conditions. When glucose and lactate concentrations were identical (5.5 mmol/L), relative contributions of glucose and lactate to neuronal oxidative metabolism amounted to 21% and 79%, respectively. Results clearly indicate that when neurons are in the presence of both glucose and lactate, they preferentially use lactate as their main oxidative substrate.

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